Anticorps Monoclonal anti-PLAGL2

PLAGL2 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3C2E5

N° de cat : 68606-1-PBS

Synonymes

ZNF900, Pleiomorphic adenoma-like protein 2, KIAA0198, 3C2E5

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Galerie de données de validation

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  WB analysis using 68606-1-Ig (same clone as 68606-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68606-1-Ig (PLAGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68606-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit testis using 68606-1-Ig (same clone as 68606-1-PBS)

  rabbit testis tissue were subjected to SDS PAGE followed by western blot with 68606-1-Ig (PLAGL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68606-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 68606-1-Ig (same clone as 68606-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PLAGL2 Monoclonal antibody (68606-1-Ig, Clone:3C2E5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68606-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68606-1-PBS cible PLAGL2 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin

• Réactivité: Humain, Lapin
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PLAGL2 Protéine recombinante Ag30227
• Nom complet: pleiomorphic adenoma gene-like 2
• Masse moléculaire calculée: 496 aa, 55 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC023655
• Symbole du gène: PLAGL2
• Identification du gène (NCBI): 5326
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PLAGL2, also named KIAA0198, belongs to the krueppel C2H2-type zinc-finger protein family. It shows weak transcriptional activatory activity. PLAGL2, a proto-oncogene, is implicated in a variety of cancers including acute myeloid leukemia (AML), malignant glioma, colon cancer, and lung adenocarcinoma. PLAGL2 can function as a tumor suppressor by initiating cell cycle arrest and apoptosis. The calculated molecular weight of PLAGL2 is 55 kDa, but the SUMO1-modifIed form is 68 kDa (PMID: 30535429).

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