Anticorps Polyclonal de lapin anti-uPA/Urokinase

• Phare
• Validé par KD/KO

uPA/Urokinase Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

37

N° de cat : 17968-1-AP

Synonymes

ATF, PLAU, U plasminogen activator, uPA, uPA/Urokinase, URK

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Galerie de données de validation

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  WB analysis using 17968-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17968-1-AP (uPA/Urokinase antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17968-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17968-1-AP (uPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 17968-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 17968-1-AP (uPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17968-1-AP

  IP Result of anti-uPA (IP:17968-1-AP, 4ug; Detection:17968-1-AP 1:1000) with HeLa cells lysate 2800ug.

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  IHC staining of human breast cancer using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17968-1-AP (uPA/Urokinase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17968-1-AP (uPA/Urokinase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17968-1-AP (uPA/Urokinase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17968-1-AP (uPA/Urokinase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 17968-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 17968-1-AP

  Immunofluorescent analysis of HeLa cells using 17968-1-AP (uPA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules A375, cellules HeLa, cellules LNCaP, tissu cérébral humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cutané humain, tissu de cancer de la prostate humain, tissu ovarien humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 29 publications below
• IHC: See 9 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

17968-1-AP cible uPA/Urokinase dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: uPA/Urokinase Protéine recombinante Ag11813
• Nom complet: plasminogen activator, urokinase
• Masse moléculaire calculée: 431 aa, 49 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC013575
• Symbole du gène: PLAU
• Identification du gène (NCBI): 5328
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLAU(Urokinase-type plasminogen activator) is also named as uPA and belongs to the peptidase S1 family.UPA initiates a proteolytic cascade, which degrades extracellular matrix during tissue growth and remodelling.The secreted proform of UPA is activated after binding to a specific high affinity cell surface receptor. Secreted UPA is exposed to rapid inactivation in the pericellular space by plasminogen activator inhibitor-1 (PAI-1), which is stored in the extracellular matrix bound to vitronectin(PMID:9665342).It has 2 isoforms produced by alternative splicing. Defects in PLAU are the cause of Quebec platelet disorder (QPD).

Publications

Species	Application	Title
human	WB	Sci Adv; p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.; Authors - Xiaochao Tan
mouse	WB	Gastroenterology; Inhibition of Plasmin Protects Against Colitis in Mice by Suppressing Matrix Metalloproteinase 9-mediated Cytokine Release From Myeloid Cells.; Authors - Shinya Munakata
mouse	IF, WB	J Exp Med; PDGF-D activation by macrophage-derived uPA promotes AngII-induced cardiac remodeling in obese mice.; Authors - Yu-Wen Cheng
human	IHC	Aging (Albany NY); Tertiary lymphoid structure stratifies glioma into three distinct tumor subtypes.; Authors - Xingwang Zhou
mouse	WB	Angiogenesis; PDGFD switches on stem cell endothelial commitment.; Authors - Weisi Lu
human	WB	Aging (Albany NY); NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium.; Authors - Pingping Ma