Anticorps Polyclonal de lapin anti-uPAR/CD87

uPAR/CD87 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(27)

Avis (1)

N° de cat : 10286-1-AP

Synonymes

PLAUR, CD87, MO3, U PAR, UPAR

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Galerie de données de validation

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  WB analysis using 10286-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10286-1-AP (uPAR/CD87 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 10286-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10286-1-AP (uPAR/CD87 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of U-251 using 10286-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using uPAR/CD87 antibody (10286-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules A2780, cellules U-251, cellules U-937
• Résultats positifs en IF/ICC: cellules U-251,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 8 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Informations sur le produit

10286-1-AP cible uPAR/CD87 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: uPAR/CD87 Protéine recombinante Ag0187
• Nom complet: PLAUR
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 35-70 kDa
• Numéro d’acquisition GenBank: BC002788
• Symbole du gène: uPAR
• Identification du gène (NCBI): 5329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

uPAR is a 45-65 kDa, highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodelling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)

Publications

Species	Application	Title
mouse	IF	Mol Cell; A transient transcriptional activation governs unpolarized-to-polarized morphogenesis during embryo implantation; Authors - Xuehui Lyu
mouse	WB	Nat Commun; Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.; Authors - Min Liu
mouse	WB	J Hazard Mater; Epigenetic reprogramming of HDAC2 in CA1 excitatory neurons determines Pb-induced non-spatial memory deficits; Authors - Mengmeng Wang
mouse	IHC	Cancer Res; Bcl-xL enforces a slow-cycling state necessary for survival in the nutrient-deprived microenvironment of pancreatic cancer.; Authors - Yogev Sela
human	WB	Nucleic Acids Res; Regulation of u-PAR gene expression by H2A.Z is modulated by the MEK-ERK/AP-1 pathway.; Authors - Santosh Chauhan
human	IHC	Mech Ageing Dev; Induction of epithelial cell senescence by SERPINE1 derived from fibroblasts in the amnion at parturition; Authors - Li-Jun Ling

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ruchi (Verified Customer) (07-26-2022)

IF on mouse skin

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Mouse skin

uPAR/CD87 Antibody Immunofluorescence validation (1:50 dilution) in Mouse skin (Cat no:10286-1-AP)

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