Anticorps Polyclonal de lapin anti-PLCB3

PLCB3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 21370-1-AP

Synonymes

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3, EC:3.1.4.11, Phosphoinositide phospholipase C-beta-3, Phospholipase C beta 3, Phospholipase C-beta-3

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Galerie de données de validation

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  WB analysis of MCF-7 using 21370-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 21370-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 21370-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 21370-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 21370-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 21370-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21370-1-AP (PLCB3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 21370-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21370-1-AP (PLCB3 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A431, cellules COLO 320, cellules HEK-293T, cellules NIH/3T3
• Résultats positifs en IHC: tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

21370-1-AP cible PLCB3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLCB3 Protéine recombinante Ag15845
• Nom complet: phospholipase C, beta 3 (phosphatidylinositol-specific)
• Masse moléculaire calculée: 1234 aa, 139 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC142681
• Symbole du gène: PLCB3
• Identification du gène (NCBI): 5331
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLCB3 is a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Six subfamilies of PLCs (B, G, D, E, Z and H) constitute part of ubiquitous signaling cascades that translate hormonal signals into intracellular events, leading to alternations in cell function. PLCB isoforms 1-4 are stimulated by G-protein activation (Gαq/11 and/or Gβγ). Independent of its enzymatic activity, PLCB3 inhibits the proliferation of hematopoietic stem cells (HSCs) and myeloid cells.

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