Anticorps Monoclonal anti-PLCD1

PLCD1 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D10B11

N° de cat : 67064-1-PBS

Synonymes

1D10B11, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1, EC:3.1.4.11, Phosphoinositide phospholipase C-delta-1, Phospholipase C delta 1

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Galerie de données de validation

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  WB analysis of human testis using 67064-1-Ig (same clone as 67064-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 67064-1-Ig (PLCD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67064-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67064-1-Ig (same clone as 67064-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67064-1-Ig (PLCD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67064-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 67064-1-Ig (same clone as 67064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 67064-1-Ig (PLCD1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67064-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 67064-1-Ig (same clone as 67064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 67064-1-Ig (PLCD1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67064-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 67064-1-Ig (same clone as 67064-1-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.2 ug PLCD1 Monoclonal antibody (67064-1-Ig, Clone:1D10B11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67064-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67064-1-PBS cible PLCD1 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PLCD1 Protéine recombinante Ag5567
• Nom complet: phospholipase C, delta 1
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC050382
• Symbole du gène: PLCD1
• Identification du gène (NCBI): 5333
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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