Anticorps Polyclonal de lapin anti-PLCD4

• Phare
• Validé par KD/KO

PLCD4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30133-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 30133-1-AP (same clone as 30133-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 30133-1-AP (PLCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 30133-1-AP (same clone as 30133-1-PBS)

  WB result of PLCD4 antibody (30133-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLCD4 transfected HeLa cells. This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

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  WB analysis of rat testis using 30133-1-AP (same clone as 30133-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 30133-1-AP (PLCD4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

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  WB analysis using 30133-1-AP (same clone as 30133-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 30133-1-AP (PLCD4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 30133-1-AP (same clone as 30133-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30133-1-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 30133-1-AP (same clone as 30133-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30133-1-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30133-1-PBS cible PLCD4 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLCD4 Protéine recombinante Ag32651
• Nom complet: phospholipase C, delta 4
• Masse moléculaire calculée: 85-90 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC006355
• Symbole du gène: PLCD4
• Identification du gène (NCBI): 84812
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PLCD4(1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4), also known as hPLCD4. It is highly expressed in skeletal muscle and kidney tissues. It may be an important enzyme for intracellular Ca²⁺ mobilization in the zona pellucida-induced acrosome reaction. PLCD4 cooperates with nuclear PKC to regulate liver regeneration. Overexpression of PLCD4 upregulates Erk signaling pathway and proliferation (PMID: 15140260).

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