Anticorps Recombinant de lapin anti-PLCG1

PLCG1 Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

242529A11

N° de cat : 84941-1-PBS

Synonymes

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, 242529A11, EC:3.1.4.11, Phosphoinositide phospholipase C-gamma-1, Phospholipase C gamma 1

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Galerie de données de validation

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  WB analysis using 84941-1-RR (same clone as 84941-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84941-1-RR (PLCG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84941-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01700-3

  Sandwich ELISA standard curve of MP01700-3, Human PLCG1 Recombinant Matched Antibody Pair - PBS only. 84941-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag27218. 84941-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP01700-3

  The mean PLCG1 concentration was determined to be 6.50 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

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  IHC staining of human liver cancer using 84941-1-RR (same clone as 84941-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84941-1-RR (PLCG1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84941-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 84941-1-RR (same clone as 84941-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 84941-1-RR (PLCG1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84941-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 84941-1-RR (same clone as 84941-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PLCG1 antibody (84941-1-RR, Clone: 242529A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84941-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 84941-1-RR (same clone as 84941-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PLCG1 antibody (84941-1-RR, Clone: 242529A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84941-1-PBS in a different storage buffer formulation.

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Informations sur le produit

84941-1-PBS cible PLCG1 dans les applications de WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PLCG1 Protéine recombinante Ag27218
• Nom complet: phospholipase C, gamma 1
• Masse moléculaire calculée: 149 kDa
• Symbole du gène: PLCG1
• Identification du gène (NCBI): 5335
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Phosphoinositide phospholipase C-gamma-1 (PLCG1) which belongs to the phosphoinositide-specific phospholipase C (PLC) family, is activated by many extracellular stimuli including hormones, neurotransmitters, and growth factors and modulates several cellular and physiological functions necessary for tumorigeneses such as cell survival, migration, invasion, and angiogenesis by generating inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) via hydrolysis of phosphatidylinositol 4,5-biphosphate (PIP2) (PMID: 9242915). Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (PMID: 2472218). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (PMID: 1708307). It has also been shown that PKA-mediated phosphorylation at Ser1248 is inhibitory to PLCγ1 tyrosine phosphorylation and phospholipase activity in CD3-treated Jurkat cells (PMID: 1370476), suggesting that Ser1248 may be an allosteric regulator of PLCγ1 activity.

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