Anticorps Monoclonal anti-PLCG2

• Phare
• Validé par KD/KO

PLCG2 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

2F8H5

N° de cat : 67011-1-PBS

Synonymes

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2, 2F8H5, EC:3.1.4.11, Phosphoinositide phospholipase C-gamma-2, Phospholipase C gamma 2

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Galerie de données de validation

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  WB analysis of Ramos using 67011-1-Ig (same clone as 67011-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 67011-1-Ig (PLCG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67011-1-PBS in a different storage buffer formulation.

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  WB analysis of Karpas-422 using 67011-1-Ig (same clone as 67011-1-PBS)

  Karpas-422 cells were subjected to SDS PAGE followed by western blot with 67011-1-Ig (PLCG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67011-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit spleen using 67011-1-Ig (same clone as 67011-1-PBS)

  Rabbit spleen tissue was subjected to SDS PAGE followed by western blot with 67011-1-Ig (PLCG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67011-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67011-1-Ig (same clone as 67011-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67011-1-Ig (PLCG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67011-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67011-1-Ig (same clone as 67011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67011-1-Ig (PLCG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67011-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67011-1-Ig (same clone as 67011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67011-1-Ig (PLCG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67011-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67011-1-PBS cible PLCG2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PLCG2 Protéine recombinante Ag24355
• Nom complet: phospholipase C, gamma 2 (phosphatidylinositol-specific)
• Masse moléculaire calculée: 148 kDa
• Poids moléculaire observé: 148 kDa
• Numéro d’acquisition GenBank: BC007565
• Symbole du gène: PLCG2
• Identification du gène (NCBI): 5336
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Phospholipase C-gamma2 (PLCG2) is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor.IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.

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