Anticorps Polyclonal de lapin anti-PLD3

• Phare
• Validé par KD/KO

PLD3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 17327-1-AP

Synonymes

5'-3' exonuclease PLD3, Choline phosphatase 3, EC:3.1.16.1, HindIII K4L homolog, HU K4

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Galerie de données de validation

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  WB analysis using 17327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 17327-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17327-1-AP (PLD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 17327-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17327-1-AP (PLD3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 17327-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17327-1-AP (PLD3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17327-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17327-1-AP (PLD3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 17327-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 17327-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 17327-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules L02, cellules SH-SY5Y
• Résultats positifs en IHC: tissu d'estomac humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

17327-1-AP cible PLD3 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLD3 Protéine recombinante Ag11306
• Nom complet: phospholipase D family, member 3
• Masse moléculaire calculée: 490 aa, 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC036327
• Symbole du gène: PLD3
• Identification du gène (NCBI): 23646
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Hum Mol Genet; Pathological characterization of a novel mouse model expressing the PD-linked CHCHD2-T61I mutation.; Authors - Teresa R Kee
human		Cell Death Discov; A signature of enhanced lipid metabolism, lipid peroxidation and aldehyde stress in therapy-induced senescence.; Authors - Amy C Flor
mouse	WB,IF	ACS Appl Mater Interfaces; FRET Nanosensor Based on DNA Tetrahedron for Visualizing PLD3 Fluctuation in Mouse Models of Alzheimer's Disease; Authors - Shuyan Wen

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