Anticorps Polyclonal de lapin anti-PLD3

• Phare
• Validé par KD/KO

PLD3 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 17327-1-AP

Synonymes

Choline phosphatase 3, HindIII K4L homolog, HU K4, Phospholipase D3, PLD 3, PLD3

---

Galerie de données de validation

• 

  WB analysis using 17327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17327-1-AP (PLD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse brain using 17327-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 17327-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 17327-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PLD3 antibody (17327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules SH-SY5Y
• Résultats positifs en IF: tissu cérébral de souris, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

17327-1-AP cible PLD3 dans les applications de WB, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLD3 Protéine recombinante Ag11306
• Nom complet: phospholipase D family, member 3
• Masse moléculaire calculée: 490 aa, 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC036327
• Symbole du gène: PLD3
• Identification du gène (NCBI): 23646
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Hum Mol Genet; Pathological characterization of a novel mouse model expressing the PD-linked CHCHD2-T61I mutation.; Authors - Teresa R Kee
human	FC	Cell Death Discov; A signature of enhanced lipid metabolism, lipid peroxidation and aldehyde stress in therapy-induced senescence.; Authors - Amy C Flor