Anticorps Polyclonal de lapin anti-PLEC

PLEC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 29170-1-AP

Synonymes

PLEC1, Plectin, PLTN, Plectin 1, PLEC1b

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Galerie de données de validation

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  WB analysis using 29170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29170-1-AP (PLEC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 29170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29170-1-AP (PLEC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 29170-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PLEC antibody (29170-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu cérébral de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

29170-1-AP cible PLEC dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLEC Protéine recombinante Ag29418
• Nom complet: plectin 1, intermediate filament binding protein 500kDa
• Masse moléculaire calculée: 532 kDa
• Poids moléculaire observé: 510 kDa
• Numéro d’acquisition GenBank: NM_201380
• Symbole du gène: Plectin
• Identification du gène (NCBI): 5339
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Plectin is a large (≥500-kDa) protein that is normally expressed in various tissues including skin, muscle, and brain. It binds to a number of cytoskeletal proteins including microtubules and intermediate filaments and is involved in establishment and dynamic modulation of the cytoskeletal network. In normal cells, it plays a crucial role in cytoskeleton network organization. In keratinocytes, plectin is concentrated at the basal surface, where it links intermediate filaments to the cytoplasmic domain of transmembrane glycoproteins such as integrin beta-4. (PMID: 24218614, PMID: 28281696, PMID: 23750011)

Publications

Species	Application	Title
human	WB,CoIP	Cell Death Dis; Epoxy metabolites of linoleic acid promote the development of breast cancer via orchestrating PLEC/NFκB1/CXCL9-mediated tumor growth and metastasis; Authors - Kai-Di Ni

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marina (Verified Customer) (03-22-2024)

Samples are two tumour (glioblastoma) cell lines (TC1, TC2). Gradient gel 4-15 %. Dilution 1:2000, primary antibody incubation 4ºC overnight, secondary antibody incubation 1 h room temperature. The blot was incubated with anti-plectin antibody after incubation with anti-spectrin beta chain (ref. 67978-1), stripping and re-blocking. The bands appear at the expected size of the target protein plectin (exposure 2 minutes).

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human tumour cell lines

PLEC Antibody Western Blot validation (1:2000 dilution) in Human tumour cell lines (Cat no:29170-1-AP)

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