Anticorps Polyclonal de lapin anti-PLEC

PLEC Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29170-1-PBS

Synonymes

PLEC1, Plectin, PLTN, Plectin 1, PLEC1b

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Galerie de données de validation

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  WB analysis using 29170-1-AP (same clone as 29170-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29170-1-AP (PLEC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29170-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 29170-1-AP (same clone as 29170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29170-1-AP (PLEC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29170-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 29170-1-AP (same clone as 29170-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PLEC antibody (29170-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29170-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29170-1-PBS cible PLEC dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLEC Protéine recombinante Ag29418
• Nom complet: plectin 1, intermediate filament binding protein 500kDa
• Masse moléculaire calculée: 532 kDa
• Poids moléculaire observé: 510 kDa
• Numéro d’acquisition GenBank: NM_201380
• Symbole du gène: Plectin
• Identification du gène (NCBI): 5339
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Plectin is a large (≥500-kDa) protein that is normally expressed in various tissues including skin, muscle, and brain. It binds to a number of cytoskeletal proteins including microtubules and intermediate filaments and is involved in establishment and dynamic modulation of the cytoskeletal network. In normal cells, it plays a crucial role in cytoskeleton network organization. In keratinocytes, plectin is concentrated at the basal surface, where it links intermediate filaments to the cytoplasmic domain of transmembrane glycoproteins such as integrin beta-4. (PMID: 24218614, PMID: 28281696, PMID: 23750011)

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