Anticorps Recombinant de lapin anti-PLEC

PLEC Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240891A10

N° de cat : 83898-1-RR

Synonymes

PLEC1, Plectin, PLTN, Plectin 1, PLEC1b

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Galerie de données de validation

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  WB analysis of A431 using 83898-1-RR

  A431 cells were subjected to SDS PAGE followed by western blot with 83898-1-RR (PLEC antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83898-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83898-1-RR (PLEC antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 83898-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLEC antibody (83898-1-RR, Clone: 240891A10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83898-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83898-1-RR against Human PLEC were performed. The affinity constant is 52.7 pM.

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Applications testées

• Résultats positifs en WB: cellules HaCaT, cellules A431, cellules HEK-293
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83898-1-RR cible PLEC dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PLEC Protéine recombinante Ag29418
• Nom complet: plectin 1, intermediate filament binding protein 500kDa
• Masse moléculaire calculée: 532 kDa
• Poids moléculaire observé: 510 kDa
• Numéro d’acquisition GenBank: NM_201380
• Symbole du gène: Plectin
• Identification du gène (NCBI): 5339
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Plectin is a large (≥500-kDa) protein that is normally expressed in various tissues including skin, muscle, and brain. It binds to a number of cytoskeletal proteins including microtubules and intermediate filaments and is involved in establishment and dynamic modulation of the cytoskeletal network. In normal cells, it plays a crucial role in cytoskeleton network organization. In keratinocytes, plectin is concentrated at the basal surface, where it links intermediate filaments to the cytoplasmic domain of transmembrane glycoproteins such as integrin beta-4. (PMID: 24218614, PMID: 28281696, PMID: 23750011)

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