Anticorps Polyclonal de lapin anti-PLEKHM1

PLEKHM1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 16202-1-AP

Synonymes

AP162, OPTB6, PH domain-containing family M member 1, Pleckstrin homology domain-containing family M member 1

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Galerie de données de validation

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  WB analysis of ROS1728 using 16202-1-AP

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 16202-1-AP (PLEKHM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 16202-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16202-1-AP (PLEKHM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16202-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16202-1-AP (PLEKHM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 16202-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 16202-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16202-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 16202-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 16202-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16202-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules ROS1728, tissu hépatique de souris
• Résultats positifs en IHC: tissu rénal de souris, tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

16202-1-AP cible PLEKHM1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLEKHM1 Protéine recombinante Ag9145
• Nom complet: pleckstrin homology domain containing, family M (with RUN domain) member 1
• Masse moléculaire calculée: 117 kDa
• Poids moléculaire observé: 117 kDa
• Numéro d’acquisition GenBank: BC064361
• Symbole du gène: PLEKHM1
• Identification du gène (NCBI): 9842
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLEKHM1, also known as AP162, OPTB6, belongs to the Pleckstrin homology domain protein family. PLEKHM1 functions as a multivalent adapter protein that regulates Rab7-dependent and HOPS complex-dependent fusion events within the endolysosomal system, thereby coupling autophagic and endocytic trafficking pathways. In osteoclasts, PLEKHM1 is crucial for bone resorption by regulating the vesicle transport process and ensuring the formation of the ruffled border.

Publications

Species	Application	Title
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
mouse	WB	Nutrients; Improving Lipophagy by Restoring Rab7 Cycle: Protective Effects of Quercetin on Ethanol-Induced Liver Steatosis.; Authors - Hongkun Lin
		Cell Biol Toxicol; Downregulation of MYBL1 in endothelial cells contributes to atherosclerosis by repressing PLEKHM1-inducing autophagy; Authors - Shi-Ao Ding

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