Anticorps Polyclonal de lapin anti-Plasminogen

Plasminogen Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 17462-1-AP

Synonymes

PLG, Plasmin heavy chain A, short form, EC:3.4.21.7, Angiostatin

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Galerie de données de validation

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  WB analysis of human blood using 17462-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 17462-1-AP (Plasminogen antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 17462-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17462-1-AP (Plasminogen antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 17462-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17462-1-AP (Plasminogen antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 17462-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17462-1-AP (Plasminogen antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17462-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 17462-1-AP (Plasminogen antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu sanguin humain, tissu placentaire humain
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

17462-1-AP cible Plasminogen dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Plasminogen Protéine recombinante Ag11217
• Nom complet: plasminogen
• Masse moléculaire calculée: 810 aa, 91 kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: BC060513
• Symbole du gène: Plasminogen
• Identification du gène (NCBI): 5340
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Plasminogen is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasminogen plays an important role in fibrinolysis as well as wound healing, cell migration, tissue modeling and angiogenesis. Plasminogen is synthesized in the liver, and it circulates in the blood, with a half-life of 2.2 days. Plasminogen is the precursor of plasmin, which lyses fibrin clots to fibrin degradation products (FDP) and D-dimer; the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. Defects in plasminogen are likely a cause of thrombophilia and ligneous conjunctivitis.

Publications

Species	Application	Title
		Front Microbiol; Binding of Plasminogen to Streptococcus suis Protein Endopeptidase O Facilitates Evasion of Innate Immunity in Streptococcus suis.; Authors - Yang Zhou
mouse	WB	Blood; A mechanism for hereditary angioedema with normal C1 inhibitor: an inhibitory regulatory role for the factor XII heavy chain; Authors - Ivan Ivanov
mouse	WB	Nat Commun; Fibrinolytic-deficiencies predispose hosts to septicemia from a catheter-associated UTI; Authors - Jonathan J Molina

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