Anticorps Polyclonal de lapin anti-Perilipin 1

Perilipin 1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 27716-1-AP

Synonymes

PLIN, PERI, PLIN1

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Galerie de données de validation

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  WB analysis using 27716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27716-1-AP (Perilipin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27716-1-AP (PLIN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 27716-1-AP

  IP result of anti-Perilipin 1 (IP:27716-1-AP, 4ug; Detection:27716-1-AP 1:2000) with NIH/3T3 cells lysate 1505 ug.

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  IHC staining of mouse brown adipose using 27716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 27716-1-AP (Perilipin 1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brown adipose using 27716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 27716-1-AP (Perilipin 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brown adipose using 27716-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brown adipose tissue using PLIN1 antibody (27716-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brown adipose using 27716-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brown adipose tissue using PLIN1 antibody (27716-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules 3T3-L1, cellules MCF-7, cellules NIH/3T3, tissu hépatique de souris
• Résultats positifs en IP: cellules NIH/3T3,
• Résultats positifs en IHC: tissu adipeux brun de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu adipeux brun de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

27716-1-AP cible Perilipin 1 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Perilipin 1 Protéine recombinante Ag26426
• Nom complet: perilipin
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 62-67 kDa
• Numéro d’acquisition GenBank: NM_001145311
• Symbole du gène: Perilipin 1
• Identification du gène (NCBI): 5346
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLIN1, also known as Perilipin 1, PLIN, PERI, belongs to the perilipin family. PLIN1 is a hormonally-regulated phosphoprotein that encircles the lipid storage droplet in adipocytes and modulates adipocyte lipid metabolism (PMID: 2040638). PLIN1 is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in inhibiting lipolysis. PLIN1 is detected in adipocytes of white adipose tissue, visceral adipose tissue, and mammary gland (PMID: 27832861, 9521880). The deficiency of PLIN1 causes lipodystrophic syndromes, which disables the optimal accumulation of triglycerides in adipocytes that results in abnormal deposition of lipids in tissues such as skeletal muscle and liver. The storage of lipids in the liver leads to insulin resistance and hypertriglyceridemia (PMID: 21345103).

Publications

Species	Application	Title
mouse	WB	Hum Gene Ther; Kallistatin Improves High-Fat-Induced Insulin Resistance via Epididymal Adipose Tissue-Derived Exosomes; Authors - Zi-Wei Yang
mouse,human	WB	Metabolism; Ubiquitination of cytoplasmic HMGB1 by RNF186 regulates hepatic lipophagy in non-alcoholic fatty liver disease; Authors - Jiang Du
mouse	IHC	Cells; Development of a Mouse Experimental System for the In Vivo Characterization of Bioengineered Adipose-Derived Stromal Cells; Authors - Taeko Ichise
goat	WB	Biol Reprod; NR4A1-mediated regulation of lipid droplets in progesterone synthesis in goat luteal cells†; Authors - Hao Yu
mouse	IF	J Plast Reconstr Aesthet Surg; Poloxamer 188 washing of lipoaspirate improves fat graft survival: A comparative study in nude mice; Authors - Meiling Liu
sheep	WB	Front Vet Sci; Profile of miRNAs induced during sheep fat tail development and roles of four key miRNAs in proliferation and differentiation of sheep preadipocytes; Authors - Wei Zhang

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