Anticorps Recombinant de lapin anti-Perilipin 1

Perilipin 1 Recombinant Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

240994H1

N° de cat : 83905-4-RR

Synonymes

PLIN, 240994H1, PERI, PLIN1

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Galerie de données de validation

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  WB analysis using 83905-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83905-4-RR (PLIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 83905-4-RR

  IP result of anti-Perilipin 1 (IP:83905-4-RR, 4ug; Detection:83905-4-RR 1:4000) with MCF-7 cells lysate 1240 ug.

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  Affinity and Kinetic Characterization of 83905-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83905-4-RR against Human Perilipin 1 were performed. The affinity constant is 0.617 nM.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules 3T3-L1, cellules MDA-MB-231
• Résultats positifs en IP: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

83905-4-RR cible Perilipin 1 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Perilipin 1 Protéine recombinante Ag26426
• Nom complet: perilipin
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: NM_001145311
• Symbole du gène: Perilipin 1
• Identification du gène (NCBI): 5346
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLIN1, also known as Perilipin 1, PLIN, PERI, belongs to the perilipin family. PLIN1 is a hormonally-regulated phosphoprotein that encircles the lipid storage droplet in adipocytes and modulates adipocyte lipid metabolism (PMID: 2040638). PLIN1 is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in inhibiting lipolysis. PLIN1 is detected in adipocytes of white adipose tissue, visceral adipose tissue, and mammary gland (PMID: 27832861, 9521880). The deficiency of PLIN1 causes lipodystrophic syndromes, which disables the optimal accumulation of triglycerides in adipocytes that results in abnormal deposition of lipids in tissues such as skeletal muscle and liver. The storage of lipids in the liver leads to insulin resistance and hypertriglyceridemia (PMID: 21345103).

Publications

Species	Application	Title
mouse	WB	J Cell Mol Med; Foxk2 Enhances Adipogenic Differentiation by Relying on the Transcriptional Activation of Peroxisome Proliferator-Activated Receptor Gamma; Authors - Shan Zhang

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