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Anticorps Recombinant de lapin anti-PLK1

PLK1 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240092B10

N° de cat : 83260-5-PBS

Synonymes

240092B10, EC:2.7.11.21, PLK, PLK 1, PLK-1

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using PLK1 Recombinant monoclonal antibody (83260-5-RR)

    WB analysis using 83260-5-RR (same clone as 83260-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83260-5-RR (PLK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83260-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PLK1 Recombinant monoclonal antibody (83260-5-RR)

    IF Staining of HeLa using 83260-5-RR (same clone as 83260-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PLK1 antibody (83260-5-RR, Clone: 240092B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83260-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PLK1 Recombinant monoclonal antibody (83260-5-RR)

    IF Staining of HepG2 using 83260-5-RR (same clone as 83260-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PLK1 antibody (83260-5-RR, Clone: 240092B10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 83260-5-PBS in a different storage buffer formulation.

Informations sur le produit

83260-5-PBS cible PLK1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet polo-like kinase 1 (Drosophila)
Masse moléculaire calculée 68 kDa
Poids moléculaire observé66 kDa
Numéro d’acquisition GenBankNM_005030
Symbole du gène PLK1
Identification du gène (NCBI) 5347
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Protein A purfication
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PLK1(Polo-like kinase 1), also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.The localization of Polo changes during the cell cycle. In interphase, Polo shows a mostly diffuse cytoplasmic localization. Polo becomes concentrated on centrosomes from early prophase and appears on centromeres or kinetochores from late prophase, before nuclear envelope breakdown (NEB). After anaphase onset, Polo relocalizes to the central spindle and remains enriched at the midbody ring in late stages of cytokinesis (PMID: 29167465).

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