Anticorps Recombinant de lapin anti-PLK2

PLK2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230520B2

N° de cat : 83431-1-PBS

Synonymes

PLK-2, PLK 2, hSNK, hPlk2, EC:2.7.11.21

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Galerie de données de validation

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  IF Staining of hTERT-RPE1 using 83431-1-RR (same clone as 83431-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using PLK2 antibody (83431-1-RR, Clone: 230520B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 83431-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83431-1-RR (same clone as 83431-1-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Plk2 Recombinant Antibody (83431-1-RR, Clone:230520B2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83431-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83431-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83431-1-RR against Human PLK2 were performed. The affinity constant is 1.53 nM.

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Informations sur le produit

83431-1-PBS cible PLK2 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PLK2 Protéine recombinante Ag29837
• Nom complet: polo-like kinase 2 (Drosophila)
• Masse moléculaire calculée: 685 aa, 78 kDa
• Numéro d’acquisition GenBank: BC013879
• Symbole du gène: PLK2
• Identification du gène (NCBI): 10769
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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