Anticorps Polyclonal de lapin anti-PLK3

PLK3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30486-1-PBS

Synonymes

PLK 3, FNK, FGF-inducible kinase, EC:2.7.11.21, Cytokine-inducible serine/threonine-protein kinase

---

Galerie de données de validation

• 

  WB analysis using 30486-1-AP (same clone as 30486-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30486-1-AP (PLK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30486-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 30486-1-AP (same clone as 30486-1-PBS)

  IP result of anti-PLK3 (IP:30486-1-AP, 4ug; Detection:30486-1-AP 1:500) with HeLa cells lysate 1750 ug. This data was developed using the same antibody clone with 30486-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 30486-1-AP (same clone as 30486-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 30486-1-AP (PLK3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30486-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

30486-1-PBS cible PLK3 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLK3 Protéine recombinante Ag33186
• Nom complet: polo-like kinase 3 (Drosophila)
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 72 kDa
• Numéro d’acquisition GenBank: BC013899
• Symbole du gène: PLK3
• Identification du gène (NCBI): 1263
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PLK3(Polo-like kinase 3) is also named as CNK, FNK, PRK and belongs to the protein kinase superfamily. It is involved in the regulation of DNA damage checkpoint as well as in M-phase function. Plk3 physically interacts with p53 and phosphorylates this tumor suppressor protein on serine-20, suggesting that the role of Plk3 in cell cycle progression is mediated, at least in part, through direct regulation of p53(PMID:12548019).

{{ptg:RelatedPrimaryAntibodies}}