Anticorps Monoclonal anti-PLOD2

• Phare
• Validé par KD/KO

PLOD2 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgA

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

1H9E1

N° de cat : 66342-1-Ig

Synonymes

1H9E1, EC:1.14.11.4, Lysyl hydroxylase 2, PLOD2/LH2, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2

---

Galerie de données de validation

• 

  WB analysis of THLE-2 using 66342-1-Ig

  WB result of PLOD2 antibody (66342-1-Ig; 1:15000; room temperature for 1.5 hours) with wild-type and PLOD2 knockout THLE-2 cells.

• 

  WB analysis using 66342-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66342-1-Ig (PLOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66342-1-Ig

  WB result of PLOD2 antibody (66342-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLOD2 transfected HEK-293 cells.

• 

  WB analysis of DU 145 using 66342-1-Ig

  DU 145 cells were subjected to SDS PAGE followed by western blot with 66342-1-Ig (PLOD2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66342-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66342-1-Ig (PLOD2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66342-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66342-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66342-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66342-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules DU 145, cellules 4T1, cellules A431, cellules A549, cellules HEK293, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 3 publications below

Informations sur le produit

66342-1-Ig cible PLOD2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgA
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PLOD2 Protéine recombinante Ag5779
• Nom complet: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
• Masse moléculaire calculée: 758 aa, 85 kDa
• Poids moléculaire observé: 87 kDa
• Numéro d’acquisition GenBank: BC037169
• Symbole du gène: PLOD2
• Identification du gène (NCBI): 5352
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Chromatographie d'affinité thiophilique
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy.(PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.

Publications

Species	Application	Title
human	WB,IHC	Front Cell Dev Biol; Identification and Validation of a Nine-Gene Amino Acid Metabolism-Related Risk Signature in HCC.; Authors - Yajuan Zhao
human	WB	J Invest Dermatol; Loss of RhoE function in dermatofibroma promotes disorganized dermal fibroblast extracellular matrix and increased integrin activation; Authors - Sofia Endzhievskaya ; KD Validated
human	WB,IHC	Cell Death Dis; Hypoxia-induced PLOD2 promotes clear cell renal cell carcinoma progression via modulating EGFR-dependent AKT pathway activation; Authors - Tao Liu ; KD Validated
human	WB	Mol Ther; Targeting RORγ inhibits the growth and metastasis of hepatocellular carcinoma; Authors - Qianqian Liu
human	IHC	Aging (Albany NY); Systematic characterization of the expression, prognosis and immune characteristics of PLOD family genes in breast cancer; Authors - Dan-Dan Wang
human	WB	Commun Biol; Hypoxia-induced circPLOD2a/b promotes the aggressiveness of glioblastoma by suppressing XIRP1 through binding to HuR; Authors - Aixin Yu

{{ptg:RelatedPrimaryAntibodies}}