Anticorps Polyclonal de lapin anti-PLOD3

• Phare
• Validé par KD/KO

PLOD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(36)

N° de cat : 11027-1-AP

Synonymes

Galactosylhydroxylysine-glucosyltransferase, LH3, Lysyl hydroxylase 3, Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3, Procollagen galactosyltransferase

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Galerie de données de validation

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  WB analysis using 11027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 11027-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11027-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11027-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11027-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 11027-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11027-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11027-1-AP (PLOD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 11027-1-AP

  IP result of anti-PLOD3 (IP:11027-1-AP, 3ug; Detection:11027-1-AP 1:500) with HepG2 cells lysate 6000ug.

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  IHC staining of human pancreas cancer using 11027-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11027-1-AP (PLOD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11027-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11027-1-AP (PLOD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 11027-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11027-1-AP (PLOD3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 11027-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11027-1-AP (PLOD3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 11027-1-AP

  Immunofluorescent analysis of HepG2 cells using 11027-1-AP (PLOD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HepG2 using 11027-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PLOD3 antibody (11027-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules PC-3, tissu placentaire de souris, tissu placentaire humain
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 26 publications below
• IHC: See 7 publications below
• IF: See 10 publications below
• IP: See 1 publications below

Informations sur le produit

11027-1-AP cible PLOD3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Drosophile, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLOD3 Protéine recombinante Ag1480
• Nom complet: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
• Masse moléculaire calculée: 738 aa, 85 kDa
• Poids moléculaire observé: 80-85 kDa
• Numéro d’acquisition GenBank: BC011674
• Symbole du gène: PLOD3
• Identification du gène (NCBI): 8985
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.

Publications

Species	Application	Title
human	WB	Sci Adv; p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.; Authors - Xiaochao Tan
		Nat Commun; Molecular architecture of the multifunctional collagen lysyl hydroxylase and glycosyltransferase LH3.; Authors - Luigi Scietti
human	WB,IF	Nat Commun; Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis.; Authors - Blerida Banushi
human	WB	Am J Hum Genet; A connective tissue disorder caused by mutations of the lysyl hydroxylase 3 gene.; Authors - Salo Antti M AM
		Matrix Biol; Antibodies and methods for immunohistochemistry of extracellular matrix proteins.; Authors - Steffen Rickelt
mouse	WB	PLoS Genet; Abnormal type I collagen post-translational modification and crosslinking in a cyclophilin B KO mouse model of recessive osteogenesis imperfecta.; Authors - Wayne A Cabral

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