Anticorps Monoclonal anti-PLOD3

• Phare
• Validé par KD/KO

PLOD3 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

2G12B8

N° de cat : 60058-1-Ig

Synonymes

2G12B8, Galactosylhydroxylysine-glucosyltransferase, LH3, Lysyl hydroxylase 3, Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3

---

Galerie de données de validation

• 

  WB analysis using 60058-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of human placenta using 60058-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 60058-1-Ig

  WB result of PLOD3 antibody (60058-1-Ig, 1:2000) with si-Control and si-PLOD3 transfected HepG2 cells.

• 

  WB analysis using 60058-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 60058-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 60058-1-Ig

  IP result of anti-PLOD3 (IP:60058-1-Ig, 5ug; Detection:60058-1-Ig 1:500) with HeLa cells lysate 1400ug.

• 

  IHC staining of human pancreas cancer using 60058-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60058-1-Ig(PLOD3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 60058-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60058-1-Ig(PLOD3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 60058-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLOD3 antibody (60058-1-Ig, Clone: 2G12B8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 60058-1-Ig

  Immunofluorescent analysis of HeLa cells using 60058-1-Ig (PLOD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

• 

  Affinity and Kinetic Characterization of 60058-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 60058-1-Ig against Human PLOD3 were performed. The Affinity Constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules 4T1, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules K-562, cellules LNCaP, cellules NIH/3T3, cellules PC-3, tissu placentaire humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

60058-1-Ig cible PLOD3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PLOD3 Protéine recombinante Ag1480
• Nom complet: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
• Masse moléculaire calculée: 738 aa, 85 kDa
• Poids moléculaire observé: 80-85 kDa
• Numéro d’acquisition GenBank: BC011674
• Symbole du gène: PLOD3
• Identification du gène (NCBI): 8985
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.

Publications

Species	Application	Title
human	WB	PLoS One; Lysyl Hydroxylase 3 Localizes to Epidermal Basement Membrane and Is Reduced in Patients with Recessive Dystrophic Epidermolysis Bullosa.; Authors - Stephen A Watt
mouse	WB	Matrix Biol Plus; Use of osteoblast-derived matrix to assess the influence of collagen modifications on cancer cells.; Authors - Neus Bota-Rabassedas
mouse	WB	JCI Insight; Transcriptional control of a collagen deposition and adhesion process that promotes lung adenocarcinoma growth and metastasis.; Authors - Xiaochao Tan

{{ptg:RelatedPrimaryAntibodies}}