Anticorps Polyclonal de lapin anti-PLRG1

• Phare
• Validé par KD/KO

PLRG1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11914-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of K-562 using 11914-1-AP (same clone as 11914-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11914-1-AP (PLRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11914-1-AP (same clone as 11914-1-PBS)

  WB result of PLRG1 antibody (11914-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLRG1 transfected HEK-293 cells. This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11914-1-AP (same clone as 11914-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11914-1-AP (PLRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

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  WB analysis of BxPC-3 using 11914-1-AP (same clone as 11914-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11914-1-AP (PLRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 11914-1-AP (same clone as 11914-1-PBS)

  IP result of anti-PLRG1 (IP:11914-1-AP, 4ug; Detection:11914-1-AP 1:1000) with HEK-293 cells lysate 1400ug. This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 11914-1-AP (same clone as 11914-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11914-1-AP (PLRG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 11914-1-AP (same clone as 11914-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11914-1-AP (PLRG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11914-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11914-1-PBS cible PLRG1 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLRG1 Protéine recombinante Ag2527
• Nom complet: pleiotropic regulator 1 (PRL1 homolog, Arabidopsis)
• Masse moléculaire calculée: 514 aa, 57 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC020786
• Symbole du gène: PLRG1
• Identification du gène (NCBI): 5356
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PLRG1, identifed as a component of the CDC5L complex, is one spliceosomal protein highly conserved across species. It has been shown to be essential for pre-mRNA splicing in yeast and humans.

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