Anticorps Polyclonal de lapin anti-PLS3

PLS3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 12917-1-AP

Synonymes

T-plastin, T-fimbrin, T plastin, Plastin-3, Plastin 3

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Galerie de données de validation

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  WB analysis using 12917-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12917-1-AP (PLS3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 12917-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12917-1-AP (PLS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 12917-1-AP

  IP result of anti-PLS3 (IP:12917-1-AP, 4ug; Detection:12917-1-AP 1:500) with A431 cells lysate 2640 ug.

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  IHC staining of human skin cancer using 12917-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12917-1-AP (PLS3 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 12917-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12917-1-AP (PLS3 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 12917-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using PLS3 antibody (12917-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 12917-1-AP

  Immunofluorescent analysis of A431 cells using 12917-1-AP (PLS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules HepG2, cellules NIH/3T3
• Résultats positifs en IP: cellules A431,
• Résultats positifs en IHC: tissu de cancer de la peau humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

12917-1-AP cible PLS3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLS3 Protéine recombinante Ag3574
• Nom complet: plastin 3 (T isoform)
• Masse moléculaire calculée: 630 aa, 71 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC039049
• Symbole du gène: PLS3
• Identification du gène (NCBI): 5358
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLS3, also named T-plastin and Plastin-3, is an actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It plays an important role in leukocyte function. L-plastin plays an important role in leukocyte function, and T-plastin is possibly involved in DNA repair. Both T- and L-plastin are implicated in several diseases, and L-plastin is considered to be a valuable marker for cancer (PMID: 15960882).

Publications

Species	Application	Title
mouse	WB	Nat Commun; Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development.; Authors - Jasmin Morandell

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Gaurav (Verified Customer) (02-06-2024)

Excellent antibody with expected molecular weight at right position. Works very well in knock out samples of Smooth Muscle Cells. Works even better at room temperature also. Very stable antibody.

• Applications: Western Blot, Immunoprecipitation, Cell culture
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Aorta, Placenta

PLS3 Antibody Western Blot,Immunoprecipitation,Cell culture validation (1:5000 dilution) in Aorta, Placenta (Cat no:12917-1-AP)

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