Anticorps Polyclonal de lapin anti-PLSCR1

• Phare
• Validé par KD/KO

PLSCR1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 11582-1-AP

Synonymes

MMTRA1B, phospholipid scramblase 1, PL scramblase 1, PLSCR1

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Galerie de données de validation

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  WB analysis using 11582-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11582-1-AP

  WB result of PLSCR1 antibody (11582-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLSCR1 transfected HEK-293 cells.

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  WB analysis using 11582-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11582-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11582-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11582-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11582-1-AP

  Mouse lung lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11582-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 11582-1-AP

  IP Result of anti-PLSCR1 (IP:11582-1-AP, 4ug; Detection:11582-1-AP 1:500) with mouse lung tissue lysate 2800ug.

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  IHC staining of mouse spleen using 11582-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11582-1-AP (PLSCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11582-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11582-1-AP (PLSCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 11582-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11582-1-AP (PLSCR1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11582-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11582-1-AP (PLSCR1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse spleen using 11582-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11582-1-AP (PLSCR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11582-1-AP

  Immunofluorescent analysis of HeLa cells using 11582-1-AP (PLSCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 11582-1-AP

  1X10^6 HeLa cells were stained with 0.2ug PLSCR1 antibody (11582-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HepG2, tissu de muscle squelettique de souris, tissu de muscle squelettique humain, tissu placentaire humain, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu splénique de souris, tissu de cancer du pancréas humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• IP: See 2 publications below

Informations sur le produit

11582-1-AP cible PLSCR1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLSCR1 Protéine recombinante Ag2172
• Nom complet: phospholipid scramblase 1
• Masse moléculaire calculée: 318 aa, 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC021100
• Symbole du gène: PLSCR1
• Identification du gène (NCBI): 5359
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IF	J Clin Invest; Phospholipid scramblase-1 regulates innate type 2 inflammation in mouse lung via CRTH2-dependent mechanisms; Authors - Ashley Hernandez-Gutierrez
human	WB	PLoS Pathog; Phospholipid scramblase 1 interacts with influenza A virus NP, impairing its nuclear import and thereby suppressing virus replication.; Authors - Weiyu Luo
human	WB, IF	J Proteome Res; An Interactome Map Reveals Phospholipid Scramblase 1 as a Novel Regulator of Hepatitis B Virus X Protein.; Authors - Yanzhi Yuan ; KD Validated
human	WB,IF,IP	Cancer Sci; Nuclear accumulation of KPNA2 impacts radioresistance through positive regulation of the PLSCR1-STAT1 loop in lung adenocarcinoma.; Authors - Wei-Chao Liao ; KD Validated
mouse	WB	J Leukoc Biol; Phospholipid scramblase 1 is required for β2-glycoprotein I binding in hypoxia and reoxygenation-induced endothelial inflammation.; Authors - Emily Archer Slone
human	WB,IHC	Mol Med; Identification of phospholipid scramblase 1 as a biomarker and determination of its prognostic value for colorectal cancer.; Authors - Kuo Yung-Bin YB