Anticorps Recombinant de lapin anti-PLSCR1

PLSCR1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242372B2

N° de cat : 84888-4-RR

Synonymes

phospholipid scramblase 1, MMTRA1B, Mg(2+)-dependent nuclease, EC:3.1.-.-, Ca(2+)-dependent phospholipid scramblase 1

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Galerie de données de validation

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  WB analysis using 84888-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84888-4-RR (PLSCR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84888-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84888-4-RR against Human PLSCR1 were performed. The affinity constant is 0.377 nM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A549, cellules HEK-293, cellules Jurkat, cellules MCF-7, tissu placentaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84888-4-RR cible PLSCR1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PLSCR1 Protéine recombinante Ag2172
• Nom complet: phospholipid scramblase 1
• Masse moléculaire calculée: 318 aa, 35 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC021100
• Symbole du gène: PLSCR1
• Identification du gène (NCBI): 5359
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLSCR1 (Phospholipid scramblase 1) is a member of the family of membrane proteins that mediate the transbilayer movement of phospholipids ("scrambling") in a Ca2+ dependent manner, which was identified as an a type II transmembrane protein involved in the calcium-dependent, nonspecific, rapid redistribution of phospholipids. A cysteine-rich palmitoylation motif [184CCCPCC189] of PLSCR1, a thioester linkage to the sulfhydryl groups of cysteines, controls the transportation of PLSCR1 to the cell membrane or nucleus (PMID: 32292520). PLSCR1 is strongly expressed in response to interferon (IFN) treatment and viral infection (PMID: 35138119, 23487022).

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