Anticorps Polyclonal de lapin anti-PLSCR3

PLSCR3 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 28028-1-AP

Synonymes

phospholipid scramblase 3, PL scramblase 3, PLSCR3

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Galerie de données de validation

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  WB analysis using 28028-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28028-1-AP (PLSCR3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28028-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28028-1-AP (PLSCR3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 28028-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28028-1-AP (PLSCR3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28028-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28028-1-AP (PLSCR3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28028-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28028-1-AP (PLSCR3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 28028-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28028-1-AP (PLSCR3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules A431, cellules BxPC-3, tissu hépatique de rat, tissu hépatique de souris, tissu pulmonaire de rat, tissu pulmonaire de souris, tissu rénal de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

28028-1-AP cible PLSCR3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLSCR3 Protéine recombinante Ag27655
• Nom complet: phospholipid scramblase 3
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC011735
• Symbole du gène: PLSCR3
• Identification du gène (NCBI): 57048
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLSCR3, also named as PLS3, belongs to the phospholipid scramblase family. It may mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. PLSCR3 may play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. It is a target of protein kinase C-δ (PKC-δ), resides in mitochondria and plays pivotal roles in regulating apoptotic response. Phosphorylation of PLSCR3 by PKC-δ is involved in the hyperthermia-induced apoptotic signal transduction pathway in Tca8113 cells, and that Hsp27 blocks this pathway to suppress hyperthermia-induced apoptosis. (PMID:20569108)

Publications

Species	Application	Title
mouse	WB	Front Mol Neurosci; Cardiolipin externalization mediates prion protein (PrP) peptide 106-126-associated mitophagy and mitochondrial dysfunction; Authors - Dongming Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elsa (Verified Customer) (05-06-2019)

the antibody is clean and give a detectable band at the expected molecular weight. Good antibody for mouse PLSCR3.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/2500
• Cell Tissue Type: mouse microglia cell line