Anticorps Polyclonal de lapin anti-PLUNC

PLUNC Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 10413-1-AP

Synonymes

BPI fold-containing family A member 1, BPIFA1, Lung-specific protein X, LUNX, NASG

---

Galerie de données de validation

• 

  WB analysis of mouse lung using 10413-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10413-1-AP (PLUNC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 10413-1-AP

  IP result of anti-PLUNC (IP:10413-1-AP, 4ug; Detection:10413-1-AP 1:300) with mouse lung tissue lysate 3600ug.

• 

  IHC staining of mouse lung using 10413-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10413-1-AP (PLUNC antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10413-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10413-1-AP (PLUNC antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10413-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10413-1-AP (PLUNC Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 10413-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10413-1-AP (PLUNC Antibody) at dilution of 1:100 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris,
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu pulmonaire de souris, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

10413-1-AP cible PLUNC dans les applications de WB, IHC, IF, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLUNC Protéine recombinante Ag0629
• Nom complet: palate, lung and nasal epithelium associated
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC012549
• Symbole du gène: PLUNC
• Identification du gène (NCBI): 51297
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLUNC, also named as LUNX, NASG and SPURT, belongs to the BPI/LBP/Plunc superfamily and Plunc family. PLUNC may be involved in the airway inflammatory response after exposure to irritants. It is associated with tumor progression. PLUNC plays a role in innate immune responses of the upper airways.

Publications

Species	Application	Title
mouse	IF	Sci Rep; Single-cell transcriptomes reveal heterogeneity of chlorine-induced mice acute lung injury and the inhibitory effect of pentoxifylline on ferroptosis; Authors - Chen-Qian Zhao
mouse,human	WB,IHC,IF,ChIP	Cell Death Dis; USP7 inhibits the progression of nasopharyngeal carcinoma via promoting SPLUNC1-mediated M1 macrophage polarization through TRIM24; Authors - Huai Liu
mouse,human	WB	Front Immunol; CC16 drives VLA-2-dependent SPLUNC1 expression; Authors - Natalie Iannuzo
Human		J Pathol Transl Med; PLUNC downregulates the expression of PD-L1 by inhibiting the interaction of DDX17/β-catenin in nasopharyngeal carcinoma; Authors - Ranran Feng
human	WB	Med Oncol; SPLUNC1 regulates LPS-induced progression of nasopharyngeal carcinoma and proliferation of myeloid-derived suppressor cells; Authors - Ling Tang

{{ptg:RelatedPrimaryAntibodies}}