Anticorps Monoclonal anti-PLVAP

PLVAP Monoclonal Antibody for IF-P, IF-Fro, FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

IF-P, IF-Fro, FC

Conjugaison

Non conjugué

CloneNo.

MECA-32

N° de cat : 65214-1-Ig

Synonymes

MECA32, MECA-32, Panendothelial cell antigen, Plasmalemma vesicle protein 1, PV 1



Applications testées

Résultats positifs en IF-Ptissu d'intestin grêle de souris,
Résultats positifs en IF-Frotissu d'intestin grêle de souris,
Résultats positifs en cytométriecellules bEnd.3,

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:125-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:125-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65214-1-Ig cible PLVAP dans les applications de IF-P, IF-Fro, FC et montre une réactivité avec des échantillons souris

Réactivité souris
Hôte / Isotype Rat / IgG2a, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène Mouse lymph node stromal cells
Nom complet plasmalemma vesicle associated protein
Numéro d’acquisition GenBankBC013517
Symbole du gène Plvap
Identification du gène (NCBI) 84094
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 0.09% sodium azide
Conditions de stockageStore at 2-8°C. Stable for one year after shipment.

Informations générales

Plasmalemma vesicle-associated protein (PLVAP), also known as PV-1, PAL-E and MECA-32 antigen, is an endothelial cell-specific type II membrane N-glycosylated glycoprotein with a molecular weight of approximately 50-65 kDa, that forms homodimers in situ (PMID: 7626790; 30231925). The MECA-32 antibody, derived from rats immunized with murine lymph node stroma, shows high specificity for mouse endothelium in both embryonic and mature tissues (PMID: 2683264; 7626790). It recognizes most endothelial cells in the embryonic and in the adult mouse, with the exception of the brain, skeletal, and cardiac muscle, where the MECA-32 antigen has a more restricted distribution. In skeletal and cardiac muscle only small arterioles and venules express the MECA-32 antigen, while in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier (PMID: 7626790).

Protocole

Product Specific Protocols
IF protocol for PLVAP antibody 65214-1-IgDownload protocol
FC protocol for PLVAP antibody 65214-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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