Anticorps Monoclonal anti-PLVAP

PLVAP Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

Publications(1)

CloneNo.

MECA-32

N° de cat : FITC-65214

Synonymes

PV 1, Plasmalemma vesicle protein 1, Panendothelial cell antigen, MECA-32, MECA32

---

Galerie de données de validation

• 

  FC experiment of bEnd.3 using FITC-65214

  1x10^6 bEnd.3 cells were surface stained with 0.25 ug FITC Plus Anti-Mouse PLVAP (MECA-32) (FITC-65214, Clone: MECA-32) (red) or 0.25 ug FITC Plus Rat IgG2a Isotype Control (2A3) (FITC-65209, Clone: 2A3) (blue). Cells were not fixed.

---

Applications testées

• Résultats positifs en cytométrie: cellules bEnd.3,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 1 publications below

Informations sur le produit

FITC-65214 cible PLVAP dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Mouse lymph node stromal cells
• Nom complet: plasmalemma vesicle associated protein
• Numéro d’acquisition GenBank: BC013517
• Symbole du gène: Plvap
• Identification du gène (NCBI): 84094
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Plasmalemma vesicle-associated protein (PLVAP), also known as PV-1, PAL-E and MECA-32 antigen, is an endothelial cell-specific type II membrane N-glycosylated glycoprotein with a molecular weight of approximately 50-65 kDa, that forms homodimers in situ (PMID: 7626790; 30231925). The MECA-32 antibody, derived from rats immunized with murine lymph node stroma, shows high specificity for mouse endothelium in both embryonic and mature tissues (PMID: 2683264; 7626790). It recognizes most endothelial cells in the embryonic and in the adult mouse, with the exception of the brain, skeletal, and cardiac muscle, where the MECA-32 antigen has a more restricted distribution. In skeletal and cardiac muscle only small arterioles and venules express the MECA-32 antigen, while in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier (PMID: 7626790).

Publications

Species	Application	Title
mouse	FC	Cell Rep; Macrophages preserve endothelial cell specialization in the adrenal gland to modulate aldosterone secretion and blood pressure; Authors - Zheng Fan

{{ptg:RelatedPrimaryAntibodies}}