Anticorps Polyclonal de lapin anti-PLXDC2

PLXDC2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 12285-1-AP

Synonymes

plexin domain containing 2, PLXDC2, TEM7R

---

Galerie de données de validation

• 

  WB analysis of A431 using 12285-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12285-1-AP (PLXDC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12285-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12285-1-AP (PLXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 12285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12285-1-AP (PLXDC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12285-1-AP (PLXDC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12285-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12285-1-AP (PLXDC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12285-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12285-1-AP (PLXDC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

12285-1-AP cible PLXDC2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLXDC2 Protéine recombinante Ag2940
• Nom complet: plexin domain containing 2
• Masse moléculaire calculée: 480 aa, 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC012885
• Symbole du gène: PLXDC2
• Identification du gène (NCBI): 84898
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLXDC2 (Plexin-domain containing 2), also named tumor endothelial marker 7-related protein (TEM7R), is a single-pass type I membrane protein with some homology to nidogen and to plexins. PLXDC2 is located in tumor endothelium and plays a role in tumor angiogenesis. Three isoforms of PLXDC2 produced by alternative splicing have been described. Recent finding revealed that PLXDC2 is a mitogen for neural progenitors and functions as a novel regulator of the development of different brain regions, suggesting its role in coordinating proliferation and differentiation in the developing nervous system.

Publications

Species	Application	Title
human	IHC,IF	Front Cell Dev Biol; Overexpression of PLXDC2 in Stromal Cell-Associated M2 Macrophages Is Related to EMT and the Progression of Gastric Cancer.; Authors - Yiming Guan
human	WB,IHC	Cancer Res Commun; Adaptive c-Met-PLXDC2 Signaling Axis Mediates Cancer Stem Cell Plasticity to Confer Radioresistance-associated Aggressiveness in Head and Neck Cancer; Authors - Liwei Lang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Boyan (Verified Customer) (09-05-2021)

This antibody detects two bands around 55 kd, as expected molecular weight. However, neither of the bands are sensitive to deglycosylation treatment, suggesting these bands are from some other proteins other than Plxdc2. According to literature, Plxdc2 is glycosylated.

• Applications: Western Blot