Anticorps Monoclonal anti-PLXNB2

PLXNB2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G9B2

N° de cat : 67265-1-PBS

Synonymes

dJ402G11.3, KIAA0315, MM1, Nbla00445, PLEXB2, plexin B2, PLXNB2

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Galerie de données de validation

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  WB analysis using 67265-1-Ig (same clone as 67265-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

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  WB analysis of ATDC-5 using 67265-1-Ig (same clone as 67265-1-PBS)

  ATDC-5 cells were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 67265-1-Ig (same clone as 67265-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67265-1-Ig (same clone as 67265-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67265-1-Ig (same clone as 67265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67265-1-Ig (PLXNB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67265-1-Ig (same clone as 67265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67265-1-Ig (PLXNB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67265-1-PBS cible PLXNB2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PLXNB2 Protéine recombinante Ag28706
• Nom complet: plexin B2
• Masse moléculaire calculée: 205 kDa
• Poids moléculaire observé: 240 kDa
• Numéro d’acquisition GenBank: NM_012401
• Symbole du gène: PLXNB2
• Identification du gène (NCBI): 23654
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB2 is one of the three plexin-B family members which participate in axon guidance and cell migration in response to semaphorins. It has been reported that plexin B2 precursor is 240 kDa and can be cleaved into alpha subunit (~170-kDa) and beta subunit (80-kDa) (PMID: 12533544). This antibody raised against 20-466aa of human plexin B2 can recognize precursor and alpha subunit of plexin B2.

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