Anticorps Recombinant de lapin anti-PML

PML Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242564C10

N° de cat : 85048-5-PBS

Synonymes

242564C10, E3 SUMO-protein ligase PML, EC:2.3.2.-, Protein PML, RING-type E3 SUMO transferase PML

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Galerie de données de validation

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  IF Staining of HeLa using 85048-5-RR (same clone as 85048-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PML antibody (85048-5-RR, Clone: 242564C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85048-5-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 85048-5-RR (same clone as 85048-5-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug PML Recombinant antibody (85048-5-RR, Clone:242564C10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85048-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85048-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85048-5-RR against Human PML were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85048-5-PBS cible PML dans les applications de IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PML Protéine recombinante Ag15377
• Nom complet: promyelocytic leukemia
• Masse moléculaire calculée: 882 aa, 98 kDa
• Numéro d’acquisition GenBank: BC020994
• Symbole du gène: PML
• Identification du gène (NCBI): 5371
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PML, also named as MYL, RNF71 and TRIM19, is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. It functions as tumor suppressor. PML is required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. It plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. PML plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. It regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation.

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