Anticorps Monoclonal anti-PMS1

PMS1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1A6G3

N° de cat : 68413-1-PBS

Synonymes

DKFZp781M0253, FLJ98259, HNPCC3, hPMS1, PMS1, PMS1 protein homolog 1, PMSL1

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Galerie de données de validation

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  WB analysis using 68413-1-Ig (same clone as 68413-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68413-1-Ig (PMS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68413-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 68413-1-Ig (same clone as 68413-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using PMS1 antibody (68413-1-Ig, Clone: 1A6G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68413-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68413-1-PBS cible PMS1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PMS1 Protéine recombinante Ag28127
• Nom complet: PMS1 postmeiotic segregation increased 1 (S. cerevisiae)
• Poids moléculaire observé: 106 kDa
• Numéro d’acquisition GenBank: BC096331
• Symbole du gène: PMS1
• Identification du gène (NCBI): 5378
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PMS1 Homolog 1 is a member of the DNA mismatch repair enzymes (MMREs) which is to eliminate the mismatch of insertions and deletions as a consequence of DNA polymerase errors at DNA synthesis in eukaryotes. PMS1 and MLH1 could form a heterodimer MutLβ that belongs to MMREs (PMID:25619773). In the MMR system, the MutS complex recognizes the DNA mispairs firstly, and then the Mlh1-Pms1 and other associated proteins such as PCNA, RFC, ExoI were recruited, inducing Exonuclease 1 (Exo1)-dependent and -independent MMR pathways (PMID:24981171). Mutations in this gene cause hereditary nonpolyposis colorectal cancer(HNPCC), which is also known as Lynch syndrome. It was reported that PMS1 is a widely expressed protein and located in the nucleus. Alternative splicing allows for 4 isoforms of PMS1 protein.

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