Anticorps Monoclonal anti-PMS2

PMS2 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

1G4E6

Avis (1)

N° de cat : 66075-1-Ig

Synonymes

1G4E6, EC:3.1.-.-, HNPCC4, PMS1 protein homolog 2, PMS2CL

---

Galerie de données de validation

• 

  WB analysis of HeLa using 66075-1-Ig

  Vairous cells were subjected to SDS PAGE followed by western blot with 66075-1-Ig (PMS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66075-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66075-1-Ig (PMS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66075-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66075-1-Ig (PMS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

66075-1-Ig cible PMS2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PMS2 Protéine recombinante Ag12661
• Nom complet: PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
• Masse moléculaire calculée: 862 aa, 96 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC093921
• Symbole du gène: PMS2
• Identification du gène (NCBI): 5395
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS).

Publications

Species	Application	Title
human	WB	Mol Carcinog; The mechanism of RGS5 regulating gastric cancer mismatch repair protein; Authors - Zhenwei Yang
human	WB	Sci Rep; Mismatch repair proteins play a role in ATR activation upon temozolomide treatment in MGMT-methylated glioblastoma.; Authors - Sachita Ganesa
human	WB	Mol Genet Genomic Med; PMS2 germline mutation c.943C>T (p.Arg315*)-induced Lynch syndrome-associated ovarian cancer.; Authors - Xiaoqing Guo
human	WB	Cell Mol Biol Lett; MRE11A: a novel negative regulator of human DNA mismatch repair; Authors - Demin Du
human	WB	Drug Resist Updat; Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane; Authors - Xin Zhang
human	WB	Cell Rep; Phosphorylation of SIRT7 by ATM causes DNA mismatch repair downregulation and adaptive mutability during chemotherapy; Authors - Lianhui Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Daniela (Verified Customer) (03-17-2022)

Bands in WB and IF staining were both aspecific.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for WB; 1:100 for IF
• Cell Tissue Type: Gastric mouse organoids and oesophageal cancer cell line

PMS2 Antibody Western Blot,Immunofluorescence validation (1:1000 for WB; 1:100 for IF dilution) in Gastric mouse organoids and oesophageal cancer cell line (Cat no:66075-1-Ig)

{{ptg:RelatedPrimaryAntibodies}}