Anticorps Recombinant de lapin anti-PNMT

PNMT Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241111G1

N° de cat : 83979-5-RR

Synonymes

241111G1, EC:2.1.1.28, Noradrenaline N-methyltransferase, PENT, Phenylethanolamine N-methyltransferase

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Galerie de données de validation

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  WB analysis of K-562 using 83979-5-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 83979-5-RR (PNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83979-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83979-5-RR against Human PNMT were performed. The affinity constant is 8.83 pM.

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Applications testées

• Résultats positifs en WB: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83979-5-RR cible PNMT dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PNMT Protéine recombinante Ag4098
• Nom complet: phenylethanolamine N-methyltransferase
• Masse moléculaire calculée: 282 aa, 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC037246
• Symbole du gène: PNMT
• Identification du gène (NCBI): 5409
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PNMT (Phenylethanolamine N-methyltransferase) is the final enzyme in the catecholamine synthesizing cascade that converts noradrenaline to epinephrine by transferring a methyl group from S-adenosyl-L-methionine (SAM) to norepinephrine. PNMT is primarily found in the adrenal medulla, where it is responsible for the production of epinephrine. It is also present in certain areas of the brain, such as the amygdala and retina, where it may be involved in various neurophysiological functions (PMID: 17175506). Variations in the PNMT gene have been associated with Alzheimer's disease, hypertension, and other disorders (PMID: 2816488,.11514309).

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