Anticorps Monoclonal anti-PNPLA3

PNPLA3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D2B11

N° de cat : 67369-1-PBS

Synonymes

Acylglycerol O acyltransferase, Adiponutrin, ADPN, C22orf20, iPLA(2)epsilon, iPLA2 epsilon, PNPLA3

---

Galerie de données de validation

• 

  WB analysis using 67369-1-Ig (same clone as 67369-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67369-1-Ig (PNPLA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67369-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67369-1-Ig (same clone as 67369-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67369-1-Ig (PNPLA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67369-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67369-1-Ig (same clone as 67369-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67369-1-Ig (PNPLA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67369-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67369-1-Ig (same clone as 67369-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PNPLA3 antibody (67369-1-Ig, Clone: 1D2B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67369-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67369-1-PBS cible PNPLA3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PNPLA3 Protéine recombinante Ag27407
• Nom complet: patatin-like phospholipase domain containing 3
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC065195
• Symbole du gène: PNPLA3
• Identification du gène (NCBI): 80339
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PNPLA3 belongs to a family of proteins that share a domain that was first identified in patatin, a major soluble protein of potato tubers with nonspecific acyl hydrolase activity. The domain differs from classical lipases by employing a catalytic dyad (Ser-Asp), rather than a catalytic triad, to effect hydrolysis. PNPLA3 is expressed primarily in liver and adipose tissue, in which it partitions to membranes and lipid droplets. Real-time PCR of cDNA from human tissues indicated that PNPLA3 expression was highest in the liver, followed by skin and adipose tissue. PNPLA3 is expressed at the highest levels in adipose tissue of mice. The PNPLA3 protein was expected at 50-70 kDa, as observed; the additional band at approximately 130-150 kDa is a oligomer bands. (PMID: 20385813, PMID: 24931521, PMID: 23023705)

{{ptg:RelatedPrimaryAntibodies}}