Anticorps Polyclonal de lapin anti-PNPLA6

PNPLA6 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14261-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HEK-293 using 14261-1-AP (same clone as 14261-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14261-1-AP (PNPLA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14261-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 14261-1-AP (same clone as 14261-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14261-1-AP (PNPLA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14261-1-PBS in a different storage buffer formulation.

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  IP experiment using 14261-1-AP (same clone as 14261-1-PBS)

  IP result of anti-PNPLA6 (14261-1-AP for IP and Detection) with HEK-293 cells. This data was developed using the same antibody clone with 14261-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14261-1-AP (same clone as 14261-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using PNPLA6 antibody 14261-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 14261-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 14261-1-AP (same clone as 14261-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using PNPLA6 antibody 14261-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14261-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14261-1-PBS cible PNPLA6 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PNPLA6 Protéine recombinante Ag5546
• Nom complet: patatin-like phospholipase domain containing 6
• Masse moléculaire calculée: 150 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC051768
• Symbole du gène: PNPLA6
• Identification du gène (NCBI): 10908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PNPLA6(patatin-like phospholipase domain-containing protein 6) is also named as NTE(neuropathy target esterase) and belongs to the NTE family. It plays an important role in motor neuron development and implicated overexpression of BMP signaling(PMID:22996643). Defects in PNPLA6 are the cause of spastic paraplegia autosomal recessive type 39 (SPG39)(PMID:18313024). This protein has a glycosylaion site that it can be glycosylated(PMID:9576844). It has 3 isoforms produced by alternative splicing.

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