Anticorps Polyclonal de lapin anti-PNPT1

• Phare
• Validé par KD/KO

PNPT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 14487-1-AP

Synonymes

3 5 RNA exonuclease OLD35, old 35, OLD35, PNPASE, PNPase 1, PNPase old 35, PNPT1, Polynucleotide phosphorylase 1

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Galerie de données de validation

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  WB analysis using 14487-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14487-1-AP (PNPT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14487-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14487-1-AP (PNPT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14487-1-AP

  IP Result of anti-PNPT1 (IP:14487-1-AP, 4ug; Detection:14487-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 14487-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14487-1-AP (PNPT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 14487-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14487-1-AP (PNPT1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 14487-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14487-1-AP (PNPT1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 14487-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14487-1-AP (PNPT1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 14487-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14487-1-AP (PNPT1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 14487-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14487-1-AP (PNPT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain, tissu cutané humain, tissu pulmonaire humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

14487-1-AP cible PNPT1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PNPT1 Protéine recombinante Ag5888
• Nom complet: polyribonucleotide nucleotidyltransferase 1
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 86 kDa
• Numéro d’acquisition GenBank: BC053660
• Symbole du gène: PNPT1
• Identification du gène (NCBI): 87178
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.

Publications

Species	Application	Title
human	WB	Immunity; Gasdermin D permeabilization of mitochondrial inner and outer membranes accelerates and enhances pyroptosis; Authors - Rui Miao ; KD Validated
mouse	WB,IHC	Am J Hum Genet; A mutation in PNPT1, encoding mitochondrial-RNA-import protein PNPase, causes hereditary hearing loss.; Authors - von Ameln Simon S ; KO Validated
mouse	IF	Proc Natl Acad Sci U S A; EF-hand protein Ca2+ buffers regulate Ca2+ influx and exocytosis in sensory hair cells.; Authors - Tina Pangršič
mouse	WB	EMBO J; CLPP coordinates mitoribosomal assembly through the regulation of ERAL1 levels.; Authors - Karolina Szczepanowska
human	WB	PLoS Genet; RNA binding protein FXR1-miR301a-3p axis contributes to p21WAF1 degradation in oral cancer.; Authors - Mrinmoyee Majumder
mouse	IF	Front Cell Dev Biol; Differences in Calcium Clearance at Inner Hair Cell Active Zones May Underlie the Difference in Susceptibility to Noise-Induced Cochlea Synaptopathy of C57BL/6J and CBA/CaJ Mice.; Authors - Hongchao Liu