Anticorps Monoclonal anti-PNPT1

PNPT1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3F2A1

N° de cat : 68309-1-PBS

Synonymes

3'-5' RNA exonuclease OLD35, 3F2A1, EC:2.7.7.8, old 35, OLD35

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Galerie de données de validation

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  WB analysis using 68309-1-Ig (same clone as 68309-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68309-1-Ig (PNPT1 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68309-1-Ig (same clone as 68309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68309-1-Ig (PNPT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68309-1-Ig (same clone as 68309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68309-1-Ig (PNPT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 68309-1-Ig (same clone as 68309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68309-1-Ig (PNPT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68309-1-Ig (same clone as 68309-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PNPT1 antibody (68309-1-Ig, Clone: 3F2A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 68309-1-Ig (same clone as 68309-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.2 μg PNPT1 Monoclonal antibody (68309-1-Ig, Clone:3F2A1, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone:1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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  FC experiment of LNCaP using 68309-1-Ig (same clone as 68309-1-PBS)

  1x10^6 LNCaP cells were intracellularly stained with 0.2 μg PNPT1 Monoclonal antibody (68309-1-Ig, Clone:3F2A1, red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone:1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68309-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68309-1-PBS cible PNPT1 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PNPT1 Protéine recombinante Ag6290
• Nom complet: polyribonucleotide nucleotidyltransferase 1
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 86 kDa
• Numéro d’acquisition GenBank: BC053660
• Symbole du gène: PNPT1
• Identification du gène (NCBI): 87178
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.

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