Anticorps Monoclonal anti-PNPT1

PNPT1 Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3F2A1

N° de cat : CL647-68309

Synonymes

3'-5' RNA exonuclease OLD35, 3F2A1, EC:2.7.7.8, old 35, OLD35

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Galerie de données de validation

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  IF Staining of A431 using CL647-68309

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 647 PNPT1 antibody (CL647-68309, Clone: 3F2A1 ) at dilution of 1:200, CL488-phalloidin (green).

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  IF Staining of HeLa using CL647-68309

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 PNPT1 antibody (CL647-68309, Clone: 3F2A1 ) at dilution of 1:200, CL488-phalloidin (green).

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  FC experiment of HepG2 using CL647-68309

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 647 Pnpt1 Monoclonal Antibody (CL647-68309, Clone:3F2A1)(red), or 0.8 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules A431, cellules HeLa
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.8 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-68309 cible PNPT1 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PNPT1 Protéine recombinante Ag6290
• Nom complet: polyribonucleotide nucleotidyltransferase 1
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 86 kDa
• Numéro d’acquisition GenBank: BC053660
• Symbole du gène: PNPT1
• Identification du gène (NCBI): 87178
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.

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