Anticorps Polyclonal de lapin anti-POFUT1

• Phare
• Validé par KD/KO

POFUT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 14929-1-AP

Synonymes

FUT12, KIAA0180, O Fuc T, O FucT 1, O FUT, Peptide O fucosyltransferase 1, POFUT1, protein O fucosyltransferase 1

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Galerie de données de validation

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  WB analysis of HCT 116 using 14929-1-AP

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 14929-1-AP (POFUT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 14929-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 14929-1-AP (POFUT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 14929-1-AP

  IP Result of anti-POFUT1 (IP:14929-1-AP, 4ug; Detection:14929-1-AP 1:500) with HepG2 cells lysate 2400ug.

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  IHC staining of human liver cancer using 14929-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14929-1-AP (POFUT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 14929-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 14929-1-AP (POFUT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 14929-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 14929-1-AP (POFUT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 14929-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14929-1-AP (POFUT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14929-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14929-1-AP (POFUT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14929-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14929-1-AP (POFUT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 14929-1-AP

  Immunofluorescent analysis of A431 cells, using POFUT1 antibody 14929-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HUVEC using 14929-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using POFUT1 antibody (14929-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules HUVEC
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HUVEC, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Informations sur le produit

14929-1-AP cible POFUT1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POFUT1 Protéine recombinante Ag6710
• Nom complet: protein O-fucosyltransferase 1
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC000582
• Symbole du gène: POFUT1
• Identification du gène (NCBI): 23509
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

POFUT1 (protein O-fucosyltransferase 1), also known as GDP-fucose protein O-fucosyltransferase, is an enzyme that catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains and plays a crucial role in Notch signaling.

Publications

Species	Application	Title
mouse	WB	Cell; In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions.; Authors - Hongling Huang ; KO Validated
human	WB,IHC,IF	EBioMedicine; PLAGL2 and POFUT1 are regulated by an evolutionarily conserved bidirectional promoter and are collaboratively involved in colorectal cancer by maintaining stemness.; Authors - Daojiang Li ; KD Validated
human	WB	EMBO Mol Med; A spoonful of L-fucose-an efficient therapy for GFUS-CDG, a new glycosylation disorder; Authors - Ren e G Feichtinger
human	WB,IHC,IF	Cell Death Dis; POFUT1 promotes colorectal cancer development through the activation of Notch1 signaling.; Authors - Yuheng Du ; KD Validated
human	IHC, IF	Cell Death Dis; LIF upregulates poFUT1 expression and promotes trophoblast cell migration and invasion at the fetal-maternal interface.; Authors - S Liu
human	WB	Cell Prolif; Epiregulin promotes trophoblast epithelial-mesenchymal transition through poFUT1 and O-fucosylation by poFUT1 on uPA.; Authors - Xinyuan Cui