Anticorps Polyclonal de lapin anti-POLD1

• Phare
• Validé par KD/KO

POLD1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15646-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 15646-1-AP (same clone as 15646-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15646-1-AP (POLD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 15646-1-AP (same clone as 15646-1-PBS)

  IP result of anti-POLD1 (IP:15646-1-AP, 4ug; Detection:15646-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15646-1-AP (same clone as 15646-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15646-1-AP (same clone as 15646-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15646-1-AP (same clone as 15646-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15646-1-AP (same clone as 15646-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15646-1-PBS cible POLD1 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLD1 Protéine recombinante Ag8178
• Nom complet: polymerase (DNA directed), delta 1, catalytic subunit 125kDa
• Masse moléculaire calculée: 1107 aa, 124 kDa
• Poids moléculaire observé: 124 kDa
• Numéro d’acquisition GenBank: BC008800
• Symbole du gène: POLD1
• Identification du gène (NCBI): 5424
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

POLD1, also known as DNA polymerase subunit delta p125, is 1107 amino acid protein, which belongs to the DNA polymerase type-B family. POLD1 colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739). As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. A previous study demonstrated that overexpression of POLD1 is associated with an increased risk of breast cancer. (PMID: 30344713 )

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