Anticorps Recombinant de lapin anti-POLD1

POLD1 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250828E5

N° de cat : 86197-2-RR

Synonymes

3'-5' exodeoxyribonuclease, CDC2, EC:2.7.7.7, EC:3.1.11.-, POLD

---

Galerie de données de validation

• 

  WB analysis using 86197-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86197-2-RR (POLD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 86197-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLD1 antibody (86197-2-RR, Clone: 250828E5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 86197-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86197-2-RR against Human POLD1 were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules PC-12, cellules RAW 264.7
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86197-2-RR cible POLD1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: POLD1 Protéine recombinante Ag8178
• Nom complet: polymerase (DNA directed), delta 1, catalytic subunit 125kDa
• Masse moléculaire calculée: 1107 aa, 124 kDa
• Poids moléculaire observé: 124 kDa
• Numéro d’acquisition GenBank: BC008800
• Symbole du gène: POLD1
• Identification du gène (NCBI): 5424
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

POLD1, also known as DNA polymerase subunit delta p125, is 1107 amino acid protein, which belongs to the DNA polymerase type-B family. POLD1 colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739). As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. A previous study demonstrated that overexpression of POLD1 is associated with an increased risk of breast cancer. (

{{ptg:RelatedPrimaryAntibodies}}