Anticorps Polyclonal de lapin anti-POLD2

POLD2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29765-1-PBS

Synonymes

DNA polymerase delta subunit 2

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Galerie de données de validation

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  WB analysis using 29765-1-AP (same clone as 29765-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29765-1-AP (same clone as 29765-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29765-1-AP (same clone as 29765-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29765-1-AP (POLD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 29765-1-AP (same clone as 29765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 29765-1-AP (same clone as 29765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 29765-1-AP (same clone as 29765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 29765-1-AP (same clone as 29765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29765-1-AP (POLD2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 29765-1-AP (same clone as 29765-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLD2 antibody (29765-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 29765-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29765-1-PBS cible POLD2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLD2 Protéine recombinante Ag31521
• Nom complet: polymerase (DNA directed), delta 2, regulatory subunit 50kDa
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 48-51 kDa
• Numéro d’acquisition GenBank: BC000459
• Symbole du gène: POLD2
• Identification du gène (NCBI): 5425
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

POLD2, also named as DNA polymerase delta subunit 2, is a 469 amino acid protein, which belongs to the DNA polymerase delta/II small subunit family. The function of the small subunit is not yet clear. POLD2 has an essential function for the small subunit in the heterodimeric core enzyme.

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