Anticorps Recombinant de lapin anti-POLDIP2

POLDIP2 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250999B11

N° de cat : 86232-3-PBS

Synonymes

HSPC017, p38, PDIP38, POLD4, Polymerase delta-interacting protein 2

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Galerie de données de validation

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  WB analysis using 86232-3-RR (same clone as 86232-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86232-3-RR (POLDIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86232-3-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 86232-3-RR (same clone as 86232-3-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 86232-3-RR (POLDIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86232-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86232-3-RR (same clone as 86232-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLDIP2 antibody (86232-3-RR, Clone: 250999B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86232-3-PBS in a different storage buffer formulation.

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Informations sur le produit

86232-3-PBS cible POLDIP2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: POLDIP2 Protéine recombinante Ag7154
• Nom complet: polymerase (DNA-directed), delta interacting protein 2
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC000655
• Symbole du gène: POLDIP2
• Identification du gène (NCBI): 26073
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

POLDIP2(Polymerase delta-interacting protein 2) is also named as PDIP38, POLD4. It is as a unique positive regulator of Nox4 via its association with p22phox and together with Nox4, has profound effects on Rhodependent cytoskeletal remodeling. It is also as a regulator of cell division. It consists of 368 amino acids and has a predicted molecular mass of 42 kDa, with a potential signal peptide cleavage site after the first N-terminal 48 residues, which would result in a protein of 37 kDa(PMID:19574552). Endogenous human and rat POLDIP2 localized to the nucleus, plasma membrane, and cytoplasm(PMID:17623671).

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