Anticorps Recombinant de lapin anti-POLDIP2

POLDIP2 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250999B11

N° de cat : 86232-3-RR

Synonymes

HSPC017, p38, PDIP38, POLD4, Polymerase delta-interacting protein 2

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Galerie de données de validation

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  WB analysis using 86232-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86232-3-RR (POLDIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 86232-3-RR

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 86232-3-RR (POLDIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86232-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLDIP2 antibody (86232-3-RR, Clone: 250999B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86232-3-RR cible POLDIP2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: POLDIP2 Protéine recombinante Ag7154
• Nom complet: polymerase (DNA-directed), delta interacting protein 2
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC000655
• Symbole du gène: POLDIP2
• Identification du gène (NCBI): 26073
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

POLDIP2(Polymerase delta-interacting protein 2) is also named as PDIP38, POLD4. It is as a unique positive regulator of Nox4 via its association with p22phox and together with Nox4, has profound effects on Rhodependent cytoskeletal remodeling. It is also as a regulator of cell division. It consists of 368 amino acids and has a predicted molecular mass of 42 kDa, with a potential signal peptide cleavage site after the first N-terminal 48 residues, which would result in a protein of 37 kDa(PMID:19574552). Endogenous human and rat POLDIP2 localized to the nucleus, plasma membrane, and cytoplasm(PMID:17623671).

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