Anticorps Polyclonal de lapin anti-POLG2

• Phare
• Validé par KD/KO

POLG2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 10997-2-AP

Synonymes

DNA polymerase subunit gamma-2, MTPOLB, p55, PEOA4, POLGB

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Galerie de données de validation

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  WB analysis of COLO 320 using 10997-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10997-2-AP (POLG2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10997-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10997-2-AP (POLG2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 10997-2-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10997-2-AP (POLG2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10997-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10997-2-AP (POLG2 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of U-251 using 10997-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using POLG2 antibody (10997-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, tissu de côlon de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U-251,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10997-2-AP cible POLG2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLG2 Protéine recombinante Ag1424
• Nom complet: polymerase (DNA directed), gamma 2, accessory subunit
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC009194
• Symbole du gène: POLG2
• Identification du gène (NCBI): 11232
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

mtDNA is replicated by DNA polymerase gamma, which is composed of a catalytic (encoded by POLG) and an accessory subunit (encoded by POLG2). POLG2(DNA polymerase subunit gamma-2, mitochondrial) can stimulate the polymerase and exonuclease activities (PMID: 37085601). Defects in POLG2 are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 4 (PEOA4) (PMID: 16685652).Western blot result suggested that the POLG2 expressed in human fetal kidney and U-251 MG cell lines with a single band of 55kd.

Publications

Species	Application	Title
human	WB	Acta Pharm Sin B; Miriplatin-loaded liposome, as a novel mitophagy inducer, suppresses pancreatic cancer proliferation through blocking POLG and TFAM-mediated mtDNA replication; Authors - Xiaowei Wang
mouse	WB	Sci Rep; PMID: 28397817; Authors - Xinbin Zhao
human	WB	Biochim Biophys Acta Mol Cell Res; TFB2M and POLRMT are essential for mammalian mitochondrial DNA replication; Authors - Teppei Inatomi
human	WB	Mitochondrion; The accessory subunit of human DNA polymerase γ is required for mitochondrial DNA maintenance and is able to stabilize the catalytic subunit.; Authors - Yura Do ; KD Validated
human	IF	Nat Cell Biol; Mitochondrial DNA replication stress triggers a pro-inflammatory endosomal pathway of nucleoid disposal; Authors - Laura E Newman
human	WB,IHC	PLoS One; Interference with mitochondrial metabolism could serve as a potential therapeutic strategy for advanced prostate cancer; Authors - Chuang Wu

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