Anticorps Polyclonal de lapin anti-POLG2

• Phare
• Validé par KD/KO

POLG2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10997-2-PBS

Synonymes

DNA polymerase subunit gamma-2, MTPOLB, p55, PEOA4, POLGB

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Galerie de données de validation

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  WB analysis of COLO 320 using 10997-2-AP (same clone as 10997-2-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10997-2-AP (POLG2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10997-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10997-2-AP (same clone as 10997-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10997-2-AP (POLG2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10997-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 10997-2-AP (same clone as 10997-2-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10997-2-AP (POLG2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10997-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10997-2-AP (same clone as 10997-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10997-2-AP (POLG2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10997-2-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 10997-2-AP (same clone as 10997-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using POLG2 antibody (10997-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10997-2-PBS in a different storage buffer formulation.

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Informations sur le produit

10997-2-PBS cible POLG2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLG2 Protéine recombinante Ag1424
• Nom complet: polymerase (DNA directed), gamma 2, accessory subunit
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC009194
• Symbole du gène: POLG2
• Identification du gène (NCBI): 11232
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

mtDNA is replicated by DNA polymerase gamma, which is composed of a catalytic (encoded by POLG) and an accessory subunit (encoded by POLG2). POLG2(DNA polymerase subunit gamma-2, mitochondrial) can stimulate the polymerase and exonuclease activities (PMID: 37085601). Defects in POLG2 are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 4 (PEOA4) (PMID: 16685652).Western blot result suggested that the POLG2 expressed in human fetal kidney and U-251 MG cell lines with a single band of 55kd.

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