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Anticorps Polyclonal de lapin anti-POLH

POLH Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB,ELISA

Conjugaison

Non conjugué

N° de cat : 28133-1-AP

Synonymes

DNA polymerase eta, FLJ16395, FLJ21978, POLH, polymerase (DNA directed), eta, RAD30, RAD30 homolog A, RAD30A, XP V, XPV

Galerie de données de validation

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  • Western Blot (WB) analysis of BxPC-3 cells using POLH Polyclonal antibody (28133-1-AP)

    WB analysis of BxPC-3 using 28133-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using POLH Polyclonal antibody (28133-1-AP)

    WB analysis of HeLa using 28133-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using POLH Polyclonal antibody (28133-1-AP)

    WB analysis of COLO 320 using 28133-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using POLH Polyclonal antibody (28133-1-AP)

    WB analysis of HEK-293T using 28133-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Applications testées

Résultats positifs en WBcellules BxPC-3, cellules COLO 320, cellules HEK-293T, cellules HeLa

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

28133-1-AP cible POLH dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène POLH Protéine recombinante Ag27973
Nom complet polymerase (DNA directed), eta
Masse moléculaire calculée713aa,78 kDa; 414aa,46 kDa
Poids moléculaire observé 78-85 kDa
Numéro d’acquisition GenBankBC015742
Symbole du gène POLH
Identification du gène (NCBI) 5429
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Protocole

Product Specific Protocols
WB protocol for POLH antibody 28133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Katherine (Verified Customer) (10-31-2023)

The quality was good. Band was around 70kD

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: 293T
POLH Antibody Western Blot validation (1:500 dilution) in 293T (Cat no:28133-1-AP)