Anticorps Polyclonal de lapin anti-POLH

POLH Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 28133-1-AP

Synonymes

DNA polymerase eta, FLJ16395, FLJ21978, POLH, polymerase (DNA directed), eta, RAD30, RAD30 homolog A, RAD30A, XP V, XPV

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Galerie de données de validation

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  WB analysis of BxPC-3 using 28133-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 28133-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 28133-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 28133-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules COLO 320, cellules HEK-293T, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

28133-1-AP cible POLH dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLH Protéine recombinante Ag27973
• Nom complet: polymerase (DNA directed), eta
• Masse moléculaire calculée: 713aa,78 kDa; 414aa,46 kDa
• Poids moléculaire observé: 78-85 kDa
• Numéro d’acquisition GenBank: BC015742
• Symbole du gène: POLH
• Identification du gène (NCBI): 5429
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,mouse	WB	J Tradit Chin Med; Integrating network pharmacology and experimental validation to uncover the synergistic effects of Huangqi ()-Ezhu () with 5-fluorouracil in colorectal cancer models; Authors - Tan Xiying

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Katherine (Verified Customer) (10-31-2023)

The quality was good. Band was around 70kD

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: 293T

POLH Antibody Western Blot validation (1:500 dilution) in 293T (Cat no:28133-1-AP)

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